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MMI CellEctor - Capillary Cell Sorting

MMI CellEctor - Capillary Cell Sorting

Technology

The MMI CellEctor is a microscope based micro capillary single cell sorting system for rapid recognition, acquisition and deposition of single or rare cells in suspension. The system is fully motorised and computer controlled. It enables the development of user controlled protocols for aspirating and depositing cells in nanoliter volumes for the isolation of single cells in a format suitable for downstream molecular analysis with nanolitre volume reaction mixtures.

The high precision of the MMI CellPump regulates the acquisition and deposition in a variety of modes, giving the user complete control in manual and automated cell recognition, acquisition and deposition. The completely software manipulated 3D CellRobot controls the precise landing position of the capillary and generates a highly reproducible contact point.

All accessories were directly developed in consultation with MMI CellEctor users to reflect their exacting needs. They allow for the deposition of single cells onto reaction slides or directly into PCR tubes, IBIDI style chambers or microfluidic devices. User defined liquid handling programmes enable the development of dedicated cleaning and service cycles for capillary cleaning and maintenance. The MMI CellEctor is highly modular and can be mounted on numerous microscope brands from entry level, mid range to high end.

1. Cell selection and recognition

Place an aliquot of the sample of cells in suspension directly onto a standard glass slide. Visualise the cells in either bright field or fluorescence. Mark the cell of interest by clicking with the mouse.

Autodetection can be achieved with the MMI CellExplorer Software designed for the auto recognition of single cells based on their colour, morphology and relative size.

2. Ultra precise cell aspiration

A glass capillary is used to aspirate the cells of interest directly. This is controlled by a high precision 3D robotic arm and ultra precise cell pump. Both can be used in full manual mode, or semi or fully automatically. More adherent cells can be collected by reversing the position of the capillary and lowering it by a few microns to allow the cells to be released by scratching and pumping simultaneously.

3. Automated cell acquistion and deposition with full visual control

Single cells in suspension can be collected manually or automatically onto a wide range of slide based platforms including IBIDI, a PCR tube, a microfluidic or other molecular analysis device. The user gets full optical control throughout the whole workflow.

4. Molecular downstream analysis

For example: FISH, IHC, ISH, immuno fluorescence, Single Cell PCR

http://www.molecular-machines.com/single_cell_sorting_products/mmi_cellector_-_capillary_cell_sorting/technology_51

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MMI CellEctor - Capillary Cell Sorting

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