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MMI CellCut - Laser Microdissection

MMI CellCut - Laser Microdissection

LMD Technology

The MMI CellCut laser microdissection (LMD) system is designed for the quick and precise isolation of cells and tissue and so it is an essential tool for molecular pathology. A wide variety of sample types including fresh frozen, paraffin embedded, archived slides, cytospins, smears and live cells can all be used for diagnostic purposes.

The MMI CellCut enables cell selection quickly and easily directly on the touch screen. The cells of interest are marked and cut automatically using a precisely focused UV-Laser. The microdissected samples are collected for downstream analysis. This results in a pure cell population for reliable analysis and diagnosis.

The MMI CellCut is highly modular and can be mounted on numerous brands of microscope from entry level, mid range to high end, suitable for the most routine to the most complex research applications.

1. Sample preparation

The section is placed on the MMI MembraneSlide, a frame slide covered with a thin membrane that is inert and has negligible auto fluorescence. Afterwards the MMI MembraneSlide is inverted and placed onto a glass slide for protection against contamination. Now the sample is sandwiched between the membrane and the glass.

2. Easy cell selection

The cells of interest can be selected on the display using either the mouse, by freehand or predefined geometrical shapes which can be modified. Any number of cells across the slide can be identified as targets within one screening process. The stage is moving to trace the path drawn and the laser is fixed and focused from below.

3. Automated cutting

The thin laser cutting path enables a precise and gentle extraction of the selected cell at an outstanding speed. The isolated target cell is collected by lowering and lifting of the adhesive cap held from above. The sample morphology remains 100% intact.

4. Selected target

After cutting the sample can be visualised on the cap. Lysis buffer is added and the tube inverted for approx. 10 mins. The cells are now in suspension ready for downstream processing.

http://www.molecular-machines.com/single_cell_sorting_products/_mmi_cellcut_-_laser_microdissection/technology_27

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MMI CellCut - Laser Microdissection

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