Qualitative method using Polymerase Chain Reaction technique

Salmonella in fishery products – Method for qualitative analysis by Polymerase Chain Reaction

1. Scope of application

This standard specifies the Salmonella qualitative method in fishery products using the Polymerase Chain Reaction (PCR) technique.

2. References

- Rapid Identification of Salmonella serovars in feces by specific detection of virulence gens, invA and spvC, by an Enrichment broth culture multiplex PCR combination assay. (CHENG HSUN CHIU AND JONATHAN T.OU - Journal of Clinical Microbiology, p.2619-2622. Oct. 1996).

- Specific detection of Salmonella spp. by multiplex polymerase chain reaction. (Appl. Environ., Microbiol., 59 (5): 1473- 1479, 1993).

- Detection of Salmonella spp. and Listeria monocytogenes in Suspended Organic Waste by Nucleic Acid Extraction and PCR. (Appl. Environ., Microbiol. 65 (5): 2235-2377, 1999).

- Nordic commitees on food analysis (NMKL) No 71, 5th ed., 1999, Salmonella - Detection in foods.

3. Interpretation of terms