Bioline Chemistry
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Product Highlights
- Fast – streamlined protocol for reliable extraction of all miRNA species in as little as 25 minutes from a wide variety of sample types
- Specific – buffering conditions and column matrix specially developed for extraction of the miRNA population
- Efficient – excellent recovery of all small RNA species (<200 nt) from even very limiting amounts of sample
- Unbiased – column-based purification eliminates bias in the small RNA molecule population that can occur with organic solvents
- Versatile – delivers high-purity miRNA from a variety of sample types including cells, tissue, bacteria and bodily fluids.
- Convenient - optional protocol for parallel extraction of all larger RNA species thereby enabling extraction of total RNA
Product Description
The ISOLATE II miRNA Kit provides a simple, efficient column-based method for the isolation of miRNA and total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol, which has been reported to introduce significant bias in recovery of selected small RNAs.
By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of two silica-membrane purification columns, the ISOLATE II miRNA Kit provides a fast method for the purification of high-quality total RNA (on the first column) and miRNA (on the second column) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.
For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit.
The ISOLATE II miRNA Kit has been designed to deliver optimal performance in miRNA expression profiling and quantification using qPCR, microarrays, small RNA library construction for small RNA-Seq, as well as single cell assays.
Applications
- RT-qPCR
- miRNA profiling
- End-point RT-PCR
- Array analysis
- Northern blotting
- Tissue expression profiling
- Biomarker discovery
- Microarray analysis
- RNA-Seq
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