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SensiFAST™ SYBR® No-ROX One-Step Kit

SensiFAST™ SYBR® No-ROX One-Step Kit

Cat No.                                  Size

BIO-72001                              100 x 20µl Reactions

BIO-72005                              500 x 20µl Reactions

Features & Benefits

Accurate quantification - hot-start capability reduces primer-dimer formation and saves time

Sensitive - achieve excellent results from difficult and low copy targets

Rapid - optimized proprietary enzyme and unique buffer chemistry for fast cycling

Flexible - compatible with all standard and fast cycling instruments


Instrument Compatibility

Roche LightCycler® 480 platform, Bio-Rad Opticon®, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).



SensiFAST SYBR® No-ROX One-Step Kit has been developed for fast real-time RT-PCR and has been validated on all commonly used real-time instruments. SensiFAST SYBR® No-ROX One-Step Kits are formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube.

The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification. This in turn results in greater reaction sensitivity.

Through the incorporation of the latest advances in buffer chemistry and enhancers SensiFAST SYBR® No-ROX One-Step Kit produces fast, highly-specific and reproducible one-step real-time RT-PCR (Fig. 1) SensiFAST SYBR® No-ROX One-Step Kit consists of a 2x SensiFAST SYBR® No-ROX One-Step mix, plus separate reverse transcriptase andRiboSafe RNase Inhibitor.

SensiFAST SYBR® No-ROX One-Step Kit is for use with real-time PCR instruments that do not require a passive reference.



SensiFAST is a trademark of Bioline

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SensiFAST™ SYBR® No-ROX One-Step Kit

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