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SensiFAST™ SYBR® Lo-ROX One-Step Kit

SensiFAST™ SYBR® Lo-ROX One-Step Kit

Cat No.                                   Size

BIO-74001                               100 x 20µl Reactions

BIO-74005                               500 x 20µl Reactions

Features & Benefits

Accurate quantification - perfect for calculating relative gene expression from RNA samples

Ultra-sensitive - unique buffer chemistry gives superior sensitivity of low copy targets

Rapid - optimized, proprietary enzyme and buffer chemistry for fast cycling

Flexible - compatible with all standard and fast cycling instruments


Instrument Compatibility

ABI 7500, 7500 FAST, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, as well as other instruments that do not require the use of a passive reference. See our product selection table for additional information.



SensiFAST SYBR® Lo-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments. SensiFAST SYBR® Lo-ROX One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation.

This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that SensiFAST SYBR® Lo-ROX One-Step Kit produces fast, highly-specific and reproducible one-step real-time RT-PCR (Fig. 1). SensiFAST SYBR® Lo-ROX One-Step Kit consists of a 2x SensiFAST SYBR®One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST SYBR® Lo-ROX One-Step Kit contains pre-mixed ROX for optional use as a passive reference.



SensiFAST is a trademark of Bioline Reagents Ltd.

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SensiFAST™ SYBR® Lo-ROX One-Step Kit

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