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SensiFAST™ SYBR® Hi-ROX One-Step Kit (SYBR one-step qRT-PCR Master Mix )

SensiFAST™ SYBR® Hi-ROX One-Step Kit (SYBR one-step qRT-PCR Master Mix )

Cat No.                                   Size

BIO-73001                               100 x 20µl Reactions

BIO-73005                               500 x 20µl Reactions

Features & Benefits

Accurate quantification - hot-start capability reduces primer-dimer formation and saves time

Sensitive - achieve excellent results from difficult and low copy targets

Rapid - optimized proprietary enzyme and unique buffer chemistry for fast cycling

Flexible - compatible with all standard and fast cycling instruments


Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, 7900 HT FAST, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.



SensiFAST SYBR® Hi-ROX One-Step Kit have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments.

SensiFAST SYBR® Hi-ROX One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity.

The inclusion of the latest advances in buffer chemistry and enhancers also ensures that SensiFAST SYBR® Hi-ROX One-Step Kit produce fast, highly-specific and reproducible one-step rea-time RT-PCR (Fig.1). SensiFAST SYBR® Hi-ROX One-Step Kit consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST SYBR® Hi-ROX One-Step Kit contains premixed ROX for optional use as a passive reference.



SensiFAST is a trademark of Bioline Reagents Ltd.

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SensiFAST™ SYBR® Hi-ROX One-Step Kit (SYBR one-step qRT-PCR Master Mix )

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