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SensiFAST™ Probe Lo-ROX One-Step Kit

SensiFAST™ Probe Lo-ROX One-Step Kit

Cat No.                               Size

BIO-78001                           100 x 20µl Reactions

BIO-78005                           500 x 20µl Reactions

Features & Benefits

Accurate Quantification - perfect for calculating relative gene expression from RNA samples

Ultra-Sensitive - unique buffer chemistry gives superior sensitivity of low copy targets

Rapid - optimized proprietary enzyme and buffer chemistry for fast cycling

Flexible - compatible with all standard and fast cycling instruments


Instrument Compatibility

ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, (Seeproduct selection table), as well as other instruments that do not require the use of a passive reference.



SensiFAST Probe Lo-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiFAST Probe Lo-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity (Fig. 1). The advanced buffer chemistry and enhancers also gives SensiFAST Probe Lo-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe Lo-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe Lo-ROX One-Step Kit also contains premixed ROX for optional use.

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SensiFAST™ Probe Lo-ROX One-Step Kit

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