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SensiFAST™ Probe Hi-ROX One-Step Kit (One-Step RT-qPCR Master Mix)

SensiFAST™ Probe Hi-ROX One-Step Kit (One-Step RT-qPCR Master Mix)

Cat No.                                    Size

BIO-77001                                100 x 20µl Reactions

BIO-77005                                500 x 20µl Reactions

Features & Benefits

Accurate quantification - perfect for calculating relative gene expression from RNA samples

Ultra-sensitive - unique buffer chemistry gives superior sensitivity of low copy targets

Rapid - optimized proprietary enyzme and buffer chemistry for fast cycling

Flexible - compatible with all standard and fast cycling instruments

 

Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

 

Description

SensiFAST Probe Hi-ROX One-Step Kit has been optimized for fast mode on fast real-time RT-PCR instruments and fast cycling conditions on standard real-time PCR instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes.

SensiFAST Probe Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn leading to greater sensitivity (Fig. 1). The advanced buffer chemistry and enhancers also give SensiFAST Probe Hi-ROX One-Step unbeatable efficiency in multiplexing (Fig. 2). SensiFAST Probe Hi-ROX One-Step Kit consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

SensiFAST Probe Hi-ROX One-Step Kit also contains pre-mixed ROX for optional use.

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SensiFAST™ Probe Hi-ROX One-Step Kit (One-Step RT-qPCR Master Mix)

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