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SensiFAST™ HRM Kit

SensiFAST™ HRM Kit

Features & Benefits

Ultra-sensitive: accurately discriminates class 4 (A/T) SNP mutations

Rapid - enabling accurate results to be achieved faster

Highly reproducible - highly-specific, high-resolution melt analysis

Optimized - assays quickly and reliably established, even for difficult genomic loci

Instrument Compatibility

ABI 7300, 7500 FAST, Qiagen Rotor-Gene™ 6000, Eppendorf Mastercycler® ep realplex, Illumina® Eco™, Roche LightCycler®; 96 and 480, Bio-Rad Opticon®, CFX96™, CFX384™, Idaho LightScanner® 32 (LS32™), Takara Thermal Cycler Dice (TP800) (See product selection table).

Description

High Resolution Melt (HRM) curve analysis is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature. It uses intercalating dyes, highly accurate melt curves and the application of specific statistical analyses.

SensiFAST HRM has been developed using the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis, ideal for small and medium-sized genotyping projects.

The combination of our SensiFAST DNA Polymerase, buffer and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences, such asclass 4 SNPs (Fig. 1) without sequence preference. Main applications of HRM include Single Nucleotide Polymorphisms (SNPs) genotyping, epigenetics (DNA methylation analysis), zygosity testing (DNA mapping and DNA fingerprinting) and gene scanning (search for the presence of unknown variations in PCR amplicons).

Notes

SensiFAST is a trademark of Bioline Reagents Ltd.

 

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SensiFAST™ HRM Kit

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