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SensiFAST™ Genotyping Kit

SensiFAST™ Genotyping Kit

Features & Benefits

Accurate allele calling - clear allele discrimination for higher confidence genotype calling

Tight fluorescence clusters - demonstrate high reproducibility and are perfect for difficult SNPs

Faster run time - enabling more experiments per day, ideal for large genotyping projects

Designed for dual-labeled probe assays - for example TaqMan® probe-based assays


SensiFAST™ Hi-ROX Genotyping Kit and SensiFAST™ Lo-ROX Genotyping Kit

Instrument Compatibility SensiFAST™ Hi-ROX Genotyping Kit

ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus (see product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Instrument Compatibility SensiFAST™ Lo-ROX Genotyping Kit

ABI 7500, 7500 Fast, ViiA7, QuantStudio 12k Flex, Agilent Mx3000P, Mx30005P or Mx4000 (see product selection table) as well as other instruments that do not require the use of a passive reference.


SensiFAST Genotyping Kit has been developed for fast, precise and highly reproducible genotyping of sequence variants, including loci with type IV SNPs. The SensiFAST Genotyping Kit is a combination of the latest advances in buffer chemistry, together with an antibody-mediated hot-start DNA polymerase system. This produces highly-specific, ultra-sensitive real-time PCR with clear allelic discrimination and outstanding allele clustering. The SensiFAST Genotyping Kit is compatible with many dual-labeled probe assays (including TaqMan® probe based assays) and has been validated on commonly used real-time instruments.

The advanced buffer chemistry in the SensiFAST Genotyping Kit provides higher stringency and more specific binding of the allele-specific probes, resulting in much narrower probe melting temperature windows. The combination of these developments leads to wider and clearer separation of allele clusters. The kit allows robust genotype calling, enabling reproducible and accurate results. The advanced buffer also means that minimal optimization is required to achieve fast, reproducible and accurate results, ideal for high-throughput in large-scale genotyping projects.


SensiFAST is a trademark of Bioline Reagents Ltd.

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SensiFAST™ Genotyping Kit

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