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SensiFAST™ cDNA Synthesis Kit

SensiFAST™ cDNA Synthesis Kit

Features & Benefits

Reproducible – unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA

Broad dynamic range – ideal for dilute and low-copy samples

Sensitive – real-time RT-PCR analysis from as little as 1pg of starting total RNA

Unbiased cDNA synthesis – complete 5’ to 3’ RNA sequence representation

Rapid – cDNA synthesis and real-time PCR in less than 60 minutes, ideal for high throughput applications

Applications

Two-step RT-PCR

Two-step RT-qPCR

Description

SensiFAST cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis for use in real-time PCR studies (Fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR.

A reverse transcriptase and new TransAmp buffer system delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility (Fig. 2) and data accuracy, making SensiFAST cDNA Synthesis Kit ideal for working with limited samples, such as laser-microdissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions (Fig. 3). Additionally SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SYBR® Kits for very fast cDNA synthesis without compromising quality (Fig. 4), with real-time results in less than an hour.

Notes

SensiFAST and TransAMP are trademarks of Bioline.

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SensiFAST™ cDNA Synthesis Kit

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