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DNA Extraction Control 560

DNA Extraction Control 560

Cat No.                           Size

BIO-35031                       500 Reactions

BIO-35032                       2000 Reactions

DNA Extraction Control enables users of diagnostic assays to validate their extraction step. DNA Extraction Control contains a known concentration of cells that contain the control DNA sequence. Cells containing internal control DNA are "spiked" into the lysis buffer with the sample prior to DNA extraction

Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.

Features & Benefits

Simple - easy monitoring and validation of DNA extraction protocols

Specific - minimal interference with sample detection

Optimized - ideal for blood, urine and sputum samples

Sensitive - specially designed for real-time PCR assays

 

Instrument Compatibility

DNA Extraction Control 560 is suitable for use with commercially available silica-membrane DNA extraction kits and CHELEX matrices and has been tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

DNA Extraction Control 560 uses Cal Fluor® Orange 560 and is also available with Quasar®670 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.

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DNA Extraction Control 560

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