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MyTaq™ HS Red Mix

MyTaq™ HS Red Mix

Cat No.                   Size

BIO-25047               200 x 50µl Reactions

BIO-25048               1000 x 50µl Reactions

Features & Benefits

All-in-one mastermix formulations - for enhanced reproducibility

Fast - complete PCR reaction in less than 30 minutes

Antibody-based hot-start polymerase - for highest specificity

Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2

Red dye - for direct gel loading

 

Applications

Fast PCR reactions

Colony PCR

Genotyping

Multiplexing

Low-copy PCR assays

Specific amplification of difficult templates (GC-rich)

Assays with prolonged reaction set-up

 

Instrument Compatibility

Compatible with Fast and Standard PCR instruments.

 

Description

MyTaq™ HS Red Mix is a ready-to-use, direct gel load, red colored, 2x mix for fast, highly-specific hot-start PCR. MyTaq HS Red Mix is powered by antibody mediated hot-start and does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield (Fig. 1), making it ideal for high-throughput.

MyTaq HS Red Mix is designed to facilitate increased efficiency, throughput and reproducibility, making MyTaq HS Red Mix perfect for techniques such as for multiplex PCR that require the ability to define a set of common reaction parameters to all of the primer sets in the reaction, with comparable extension efficiencies, even though the amplicons are different sizes (Fig. 2).

The specially designed MyTaq Red formulation does not interfere with the PCR reaction and allows users to load samples directly onto a gel after the PCR without the need to add loading buffer, making MyTaq HS Red Mix ideal for automation. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).

MyTaq HS Red Mix only requires the addition of template, primers and water, reducing the risk of pipetting errors and contamination as well as reducing the set-up time.

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MyTaq™ HS Red Mix

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