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MyTaq™ HS Mix (Hot Start PCR Master Mix (2X))

MyTaq™ HS Mix (Hot Start PCR Master Mix (2X))

Cat No.                         Size

BIO-25045                     200 x 50µl Reactions

BIO-25046                     1000 x 50µl Reactions

Features & Benefits

All-in-one mastermix formulations - for enhance reproducibility

Fast - complete PCR reaction in less than 30 minutes

Antibody-based hot-start polymerase - for highest specificity

Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2

Available with red dye - for direct gel loading

 

Applications

Fast PCR reactions

Colony PCR

Genotyping

Multiplexing

Low-copy PCR assays

Specific amplification of difficult templates (GC-rich)

Assays with prolonged reaction set-up

 

Description

MyTaq™ HS Mix is a ready-to-use 2x mix for fast, highly-specific, hot-start PCR. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield (fig. 1), making it ideal for high-throughput.

MyTaq HS Mix contains all the reagents including MyTaq buffer, dNTPs, MgCl2, enhancers and stabilizers necessary for trouble-free PCR reaction set up. The product is supplied conveniently all-in-one tube to reduce the number of pipetting steps and to facilitate increased efficiency, throughput and reproducibility. This makes MyTaq HS Mix perfect for techniques such as multiplex PCR that require the ability to define a set of common reaction parameters to all of the primer sets in the reaction, with comparable extension efficiencies, even though the amplicons are different sizes (fig. 2).

MyTaq HS Mix enables success in multiplex PCR at the first attempt and can even be used for fast multiplex PCR, without special kits, buffers or optimization.

 

Notes

MyTaq is a trademark of Bioline.

 

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MyTaq™ HS Mix (Hot Start PCR Master Mix (2X))

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