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MyFi™ Mix

MyFi™ Mix

Cat No.                    Size

BIO-25049                100 x 50µl Reactions

BIO-25050                500 x 50µl Reactions

Features & Benefits

Convenient - ready-to-use, all-in-one 2x mastermix

New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase

Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up

High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb

Novel 5x buffer system - ensures reliability, reproducibility and convenience



TA Cloning

Long PCR

Low-copy PCR assays

PCR of difficult DNA templates (problematic GC/AT-rich genomic DNA)

High-fidelity PCR assays (library amplification)



MyFi Mix is a ready-to-use 2x mix formulation, containing all the components (including proprietary enhancers and stabilizers) necessary for trouble-free PCR reaction assembly, while delivering the same unique balance of PCR sensitivity and high-fidelity. The pre-optimized MyFi Mix formulation, supplied in a convenient single tube, reduces the number of pipetting steps improving throughput and reproducibility (Fig. 1).

MyFi Mix has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates. MyFi Mix generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi Mix provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.


MyFi is a Trademark of Bioline.

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MyFi™ Mix

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