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TRIsure™

TRIsure™

Features & Benefits

High yield - column-free extraction

Rapid - isolation of high-quality RNA, DNA or protein in 60 minutes

Convenient - perfect for a wide variety cells, tissues and bacteria

Ultra-pure - isolated material is ready for downstream applications

Applications

Purified RNA is ideal for any downstream application such as RT-PCR, one-step RT-PCR, in vitro translation, Northern blotting, RNase protection assays or dot blot hybridization

Purified DNA can be used for PCR real-time PCR and Southern blotting

Purified protein can be used for Western blotting, recovery of some enzymatic activity and some immunoprecipitation

Description

TRIsure™ allows the user to perform sequential precipitation of RNA, DNA and proteins from a single sample. After homogenizing the sample with TRIsure, the homogenate is allowed to separate into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation (fig. 1). The highly effective RNase inhibitory property of TRIsure protects the integrity of the RNA during lysis and results in the isolation of high-quality material. DNA is precipitated from the organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation.

Notes

TRIsure is a trademark of Bioline.

 

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TRIsure™

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